[George Brownlee's son, Edward George Brownlee, died in December 1995 of a brain haemorrhage. See the draft of the obituary for The Times and the Hull Daily Mail at the end of this Notebook and various other notes relating to his death.] A series of experiments setting up an RT-PCR assay for detecting a full length cRNA transcript of a synthetic 50-mer (tied vRNA panhandle) and MN-cores. Also RT-PCR for polyadenylated...

Notebook covers experiments including: All Influenza-related experiments initially confirming the sequence (by cloning, experiment 508) of the 54 transcript (see notebook 13). Experiments (with Ervin Fodor) showing size of poly(A) [32P] ATP-labelled "streak" of a transcript of T7 synthesized 49-mer vRNA (experiment 527) showing polyA was 50 >245 long, with a mid-point at 135 nucleotides...