Dunn School: Notebook 13, Oct 1994–Dec 1995
File
MS. 12364/16
Held in our offsite storage facility
[George Brownlee's son, Edward George Brownlee, died in December 1995 of a brain haemorrhage. See the draft of the obituary for The Times and the Hull Daily Mail at the end of this Notebook and various other notes relating to his death.]
A series of experiments setting up an RT-PCR assay for detecting a full length cRNA transcript of a synthetic 50-mer (tied vRNA panhandle) and MN-cores. Also RT-PCR for polyadenylated transcripts. Both assays were ultimately successful, although unpublished (see below). They provided the basis for further work by David Pritlove and Leo Poon which was published later.
A series of experiments setting up an RT-PCR assay for detecting a full length cRNA transcript of a synthetic 50-mer (tied vRNA panhandle) and MN-cores. Also RT-PCR for polyadenylated transcripts. Both assays were ultimately successful, although unpublished (see below). They provided the basis for further work by David Pritlove and Leo Poon which was published later.
- See experiment 453 for sequence of 50-mer.
- See experiment 464C for successful poly(A) product detection, a 54-mer using a phased T15A primer.
- See experiment 466 for polyA "streak" of an unphased GC clamped T20 primer.
- See experiment 485 comparing a T15 and T20 phased primer changing the PCR product from 54 to 59 long. See p. 126 where they are run side-by-side.
- See experiment 490 for RT-PCR assay for full length cRNA synthesis, successful at last.
- See experiment 492 RNase H targeting of 54-mer.
- See experiment 501 PolyA + and – purification of transcripts. Then RT-PCR of PolyA + mRNA and polyA - RNA (i.e. cRNA) separately.
Dates
- Oct 1994–Dec 1995
Extent
1 volume
Language of Materials
- English
Shelfmark
MS. 12364/16
Repository Details
Part of the Bodleian Libraries Repository
Contact:
Weston Library
Broad Street
Oxford OX1 3BG United Kingdom
specialcollections.enquiries@bodleian.ox.ac.uk
Weston Library
Broad Street
Oxford OX1 3BG United Kingdom
specialcollections.enquiries@bodleian.ox.ac.uk