Dunn School: Notebook 15, Apr 1999- Feb 2000

File

MS. 12364/18

Held in our offsite storage facility

Influenza-related experiments:

Experiments on poly(A) transcripts of 49-mer vRNA (experiment 557). Cloning showed various lengths of poly(A) tail.
Attempt at PCR and nested PCR to get evidence for poly(A)+ transcripts of 49-mer and band 8 transcripts from flu infected cells. Inconclusive.
Construction of a poly(A)+ cDNA library of full length clones from flu infected MDBK cells (experiment 606) using GC-clamped T20. Cloning showed band 8 clones were present.
List of oligos (GGB 7-GGB 25) used in this project and elsewhere are listed at the end of the notebook.

1 volume

MS. 12364/18

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