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Dunn School: Notebook 11, Sep 1993–Aug 1994

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MS. 12364/14

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All experiments relate to influenza virus - particularly to the vRNA promoter (experiments 308, 309 etc.) and its binding to MN cores, to baculovirus-expressed PB1 and Baculovirus-expressed PB2 (with Ervin Fodor). Brownlee and Fodor designed a "tied panhandle" i.e. oligo 134 for vRNA and oligo 135 for cRNA (experiments 308, 309). They also compared results with a "perfect duplex" of the vRNA promoter.

These experiments depended on the synthesis of RNA, in house, by Keith Gould.

Includes:
  1. Experiment 313: With David Pritlove and Ervin Fodor.
  2. Experiments 313, 342, 344: Convincing gel shifts.
  3. Experiment 376: Competition with yeast RNA successful.
  4. Experiment 361 etc.: North-Western blotting (e.g experiment 374 PB1>PB>NP on a molar basis). U15 binds poorly.
  5. Experiments 377, 378: Synthesis of (p)ppGomAAUACUCAAG (oligo 202, with Keith Gould, and with advice from Gordon Lowe (Chemistry and Lincoln College). Oligo 202 was a potential substrate for conversion to a 32P labelled capped oligo m7G32pppGomAAUACUCAAG to study initiation of mRNA synthesis by influenza polymerase in vitro.

Dates

  • Sep 1993–Aug 1994

Extent

1 volume

Shelfmark

MS. 12364/14

Repository Details

Part of the Bodleian Libraries Repository

Contact:
Weston Library
Broad Street
Oxford OX1 3BG United Kingdom