Notebook covers experiments including: Experiment 678: Shows both 5' & 3' strands needed for transcripts by recombinant flu polymerase. Also the polymerase trimer is required; no activity with all 3 possible dimers. Experiment 680: Globin primer gives a signal. Experiment 687: Heat stability tests on wild type and various PA mutant-containing recombinant polymerases…

Notebook covers experiments including: Many experiments attempting primer-independent synthesis with recombinant flu polymerase using short oligo 5' and 3' promoters. (Experiment 759 onwards). Most experiments were inconclusive, although many experiments suggested there was a different profile of bands when with primer-independent transcription than when a primer, ApG, was used. …