Dunn School: Notebook 18, Aug 2001-Jan 2003
Notebook covers experiments including:
- Many experiments attempting primer-independent synthesis with recombinant flu polymerase using short oligo 5' and 3' promoters. (Experiment 759 onwards). Most experiments were inconclusive, although many experiments suggested there was a different profile of bands when with primer-independent transcription than when a primer, ApG, was used.
- Recombinant polymerase prepared using tagged recombinant proteins either PA-His-tagged or PB2 TAP-tagged (experiment 755).
- Description of design and construction of control Active Site mutated (ASM) flu polymerase constructed by Tannis Jung (experiment 756).
- Aug 2001-Jan 2003