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Dunn School: Notebook 3, Oct 1983-Jul 1984

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MS. 12364/4

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Notebook covers experiments including:
  1. Experiment 208: Patient data in the hand of Peter Winship (DPhil student); also patients with haemophilia A or with fragile X.
  2. Experiment 210: Plasmids in Legionella? (with John Tobin). None detected.
  3. Experiment 213A: Karyotype of Goss hybrid (with Ted Evans).
  4. Experiment 213B: Experiments with HAE-6 hemagglutinin expressing clone (with Alain Townsend and Andrew McMichael) - also experiments 228, 229 and 232 (Southern blots) successful. 3-7 copies of HA inserted.
  5. Experiment 213C: E. coli library attempt (failed) and following.
  6. Experiment 223: RFLP analysis of carriers (theoretical).
  7. Experiment 225: Influenza NP expression in oocytes (with Coleman and Davey, Univ. Warwick). Comparison with IM Jones' (post-doctoral researcher) NP expression construct. See also experiment 230 with Ian Jones and Keith Gould [V.K is Vroni Knott, Brownlee's assistant, Joyce Huddleston having left].
  8. Experiment 227: Factor VIII pig amino acid sequence data. (Brownlee was considering cloning the factor VIII gene.)
  9. Experiment 238: E. coli cosmid libraries with pTM and pDJ (modified by DJGR, Jaspar Rees) -first experiment. Successful. (See insert on p.95 in Vroni Knottā€™s handwriting), also experiment 251A, 252, 258 (overlap 1, 2, 3 and 4) and experiment 266.
  10. Experiment 264: Use of pATX (in house derivative of pAT/PvuII/8) for cloning.
  11. Summary: Many different experiments on factor IX, the influenza HA (useful) Goss Harris hybrids (not useful), and cosmid cloning of E coli genome (useful). Have given up on sorting the X chromosome to make cosmid libraries.

Dates

  • Oct 1983-Jul 1984

Extent

1 volume

Conditions Governing Access

Closed.

Shelfmark

MS. 12364/4

Repository Details

Part of the Bodleian Libraries Repository

Contact:
Weston Library
Broad Street
Oxford OX1 3BG United Kingdom